发明名称 |
CYTOTOXIN WITH A SUBTILASE DOMAIN |
摘要 |
A member of a new class of bacterial toxin has been isolated and characterised. The bacterial toxin is of the AB5 type and is characterised in that it has a subtilase domain. It is cytotoxic to Vero cells, and toxicity in vivo in mice occurs in a number of different sites. Mutation of the serine active residue results in greater than 99% reduction in activity. The protein has been purified and antibodies have been prepared for both the A subunit and B subunits, and ELISA detection methods have been developed. The nucleic acid sequence has been determined and primers specific for the toxin have been used for a preliminary screen of a range of patient samples to ascertain the extent to which the toxin is present.
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申请公布号 |
CA2465009(A1) |
申请公布日期 |
2005.06.22 |
申请号 |
CA20042465009 |
申请日期 |
2004.05.12 |
申请人 |
ADELAIDE RESEARCH & INNOVATION PTY LTD. |
发明人 |
PATON, JAMES CLELAND;PATON, ADRIENNE WEBSTER |
分类号 |
C12N9/52;(IPC1-7):C12N15/57;A61K38/48;A61K39/02;C07K14/195;C07K16/12;C07K16/40;C12N15/31;C12P21/02;C12Q1/68;G01N33/543;G01N33/569 |
主分类号 |
C12N9/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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