| 主权项 |
1. A method of producing a recombinant antibody, or an antigen-binding fragment thereof, comprising culturing a host-cell which expresses the recombinant antibody or antigen-binding fragment thereof in a culture medium having an osmolality of about 400 mOsm/Kg or less, wherein (i) the culture medium comprises a salt selected from the group consisting of potassium at a concentration of about 70 mM or less, sodium at a concentration of about 200 mM or less, and combinations thereof, (ii) the culture medium is substantially free of one or more amino acids selected from the group consisting of alanine, arginine, aspartic acid and glutamic acid, (iii) the culture medium comprises an osmoprotectant selected from the group consisting of betaine, glycine, L-threonine, L-proline, and derivatives thereof, and (iv) fewer than about 10% of the recombinant antibody molecules in the composition have more than 4 mannose residues. |
