METHOD OF PREPARING OOCYTES, EMBRYOS, OR BLASTOCYSTS FOR CRYOPRESERVATION

摘要

An automated system and method of cryopreservation of oocytes, embryos, or blastocysts. The method entails delivering two or more solutions into a container holding oocytes, embryos, or blastocysts, and controlling the flow of the solutions to gradually change the concentration of cryoprotectants and dehydrating agents in the container to minimize shock to the oocytes, embryos or blastocysts.

主权项

1. A method of preparing oocytes, embryos or blastocysts for cryopreservation, said method comprising:
positioning one or more oocytes, embryos or blastocysts in a container; and flowing a first solution comprising a cryoprotectant over the oocytes, embryos or blastocysts by delivering the first solution into the container and removing the first solution from the container, for a first time period; while delivering the first solution into the container, initiating flow of a second solution comprising a dehydrating agent into the container; wherein the step of initiating flow of the second solution is initiated while some of the first solution is in the processing container, and accomplished to gradually increase the concentration of the dehydrating agent in the container.