| 摘要 |
<p>PURPOSE: A capillary having a coated inner wall is provided which adsorbs alternately an ionic polymer having a positive charge and ionic polymer having a negative charge to the inner wall thereof. CONSTITUTION: Contamination on an inner wall of a capillary is first removed, and in order to completely dissociate a silanol group, it is rinsed with 1N NaOH for 30 minutes and then with water for 15 minutes by using a rinsing function of a capillary electrophoresis apparatus(Beckman P/ACE 2100). It is then rinsed with a 0.4% polybrene aqueous solution for 30 minutes, followed by being allowed to stand over night. After standing over night, it is confirmed that the electrosmosis flow (eo) has been reversed by using a marker(formamide diluted solution), it is rinsed with a 5% polybrene aqueous solution for 5 minutes, followed by immediately rinsing with 2% dextran sulfate aqueous solution for 10 minutes, to produce a capillary according to the present invention.</p> |
