| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for easily and rapidly screening a gene which is important for developing a medicine by cloning a gene fragment by introducing into a competent cell by incorporating a specific PCR product into a cloning vector. SOLUTION: This method comprises the steps of (i) cleaving a vector DNA derived from a plasmid with a restriction enzyme, (ii) dephosphorylating end(s) of the cleaved DNA, (iii) obtaining a DNA fragment mixture by cleaving a chromosomal DNA derived from an object organism with the same restriction enzyme, (iv) obtaining a linked DNA mixture by carrying out ligation using the dephosphorylated DNA which was obtained in step ii and the DNA fragment mixture which was obtained in step iii, (v) amplifying the linked DNA by PCR using the linked DNA mixture as a template and arbitrary primers, and (vi) cloning the gene fragment by introducing into a competent cell by incorporating the PCR product into a cloning vector.</p> |
