IMPROVED PROTEIN SECRETION IN EUKARYOTIC CELLS

摘要

The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of an eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant alpha-1,2 mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gone, as a host for protein secretion.