Preparation and xenotransplantation of porcine islets

摘要

A subcutaneous tubes and capsules containing viable islet cells from a -20 to +20 full term gestation piglet capable of producing porcine insulin in response to glucose within a recipient mammal, and in vivo limits the egress of the islet cells and allows an abundant access to blood vessels and also containing Sertoli cells to substantially reduce or prevent immune rejection within the mammal, wherein the islets cells have been isolated from a culture supported by HAS using a collagenase and prior to containment in the tube have been exposed to nicotinamide is used in the manufacture of a medicament of treating a patient predisposed to or suffering from diabetes by xenotransplantation of the tube or capsule. Also described is a method for preparing a device suitable of delivery or implantation or xenotransplantation in a recipient mammal. (62) Divided out of 507616