Compositions for detection and analysis of polynucleotides using light harvesting multichromophores

摘要

Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PNA complementary to the target polynucleotide. The sensor PNA comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.

主权项

1. A composition comprising:
a water soluble polycationic multichromophore conjugated polymer, comprising a backbone of conjugated segments, and a signaling chromophore, wherein the composition is in an aqueous solution, and wherein a greater amount of energy is emitted from the signaling chromophore upon excitation of the conjugated polymer with incident light than from direct excitation of the signaling chromophore with incident light.