METHOD FOR PRODUCTION OF HUMAN TISSUE TYPE PLASMINOGEN ACTIVATOR

摘要

Human tissue type plasminogen activator (tPA) which is effective as a therapeutic agent is obtained at a high concentration by increasing the osmotic pressure of the cell culture medium or tPA producing medium using bicarbonate ion up to at least 350 milliosmole/liter optionally also supplementing the medium with an antiplasmin agent. Singlechain tPA is produced at a high concentration accordingly.