IL-2 TRANSMEMBRANE CONSTRUCTS

摘要

Compounds, genetic constructs, and cancer treatment methods 035 provided. Expression vectors were designed to express fusion genes including hIL-2 with 0 FcT-< transmembrane anchor derived from 0 subunit of the FC epsilon receptor. mRN 0nd the IL­2tm fusion protein was expressed in transfected RD995 tumor cells. Expression of the IL­2tm protein on the tumor A511 surface membrane was confirmed by microscopy. RD995 ce11s transfected with IL-2tm or pCMV2b (empty expression vector) were implanted subcutaneously into !3 /HEN mice. Tumors in groups of mice implanted with 10<6> or 10<5> RD995 ce11s transfected with IL-2tm grew slower than controls. Without being limited to any one theory, it is believed that selective expression >f cytokines such as IL-2 on the surface >f tumors is likely to stimulate tumor-infiltrating lymphocytes that are primed and already recognize tumor antigens, enhancing tumor recognition 0nd killing, potentially avoiding toxicity associated with known cytokine therapies.