| 发明名称 |
PURIFICATION METHOD FOR VIRUS-LIKE PARTICLES |
| 摘要 |
The present invention aims at establishing a novel means for purifying norovirus VLPs. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM. |
| 申请公布号 |
US2016168543(A1) |
申请公布日期 |
2016.06.16 |
| 申请号 |
US201414904364 |
申请日期 |
2014.05.21 |
| 申请人 |
VESIKARI Timo;BLAZEVIC Vesna;UMN PHARMA INC. |
发明人 |
OKADA Masahiro;MUKAI Akiko;NISHINO Tomonori;ARINOBU Daisuke;TO Hiroyuki;SATOH Mamoru |
| 分类号 |
C12N7/00 |
主分类号 |
C12N7/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM. |
| 地址 |
Tampere FI |
