发明名称 |
Alternative splicing variant of OATP1B3 mRNA |
摘要 |
[PROBLEM TO BE SOLVED];An object of the present invention is to provide a novel tumor marker and use thereof. In more detail, the present invention provides a novel tumor marker, a method for measuring said tumor marker and a measurement kit, a method for detecting cancer using the same, a kit for detecting cancer, a method for screening a preventive and/or therapeutic agent for cancer, as well as a medicament such as cancer vaccine.;[SOLUTION];According to the present invention, a method for measuring an alternative splicing variant of OATP1B3 mRNA in a sample to be examined is provided. Said measurement method comprises measuring mRNA comprising a nucleotide sequence represented by SEQ ID NO: 1 in the sequence listing table in a sample to be examined isolated from living organism with differentiation from a mRNA comprising a nucleotide sequence represented by SEQ ID NO: 2. Said measurement method is useful for detecting cancer or screening a preventive and/or therapeutic agent. |
申请公布号 |
US9404158(B2) |
申请公布日期 |
2016.08.02 |
申请号 |
US201113030485 |
申请日期 |
2011.02.18 |
申请人 |
National University Corporation Chiba University |
发明人 |
Furihata Tomomi;Matsumoto Sayaka;Nagai Miki;Chiba Kan |
分类号 |
C07H21/04;C12Q1/68;C07K14/47;G01N33/50;A61K39/00 |
主分类号 |
C07H21/04 |
代理机构 |
Cesari and McKenna, LLP |
代理人 |
Cesari and McKenna, LLP |
主权项 |
1. An isolated deoxyribonucleic acid satisfying the following conditions:
(1) the isolated deoxyribonucleic acid is capable of hybridizing to a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 under a stringent condition; (2) the isolated deoxyribonucleic acid does not hybridize to a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 2 under the stringent condition or all but its 3′ terminus hybridizes to the nucleic acid comprising the nucleotide sequence of SEQ ID NO: 2 under the stringent condition; and (3) 20% to 80% of the length of the isolated deoxyribonucleic acid hybridizes to exon SV in SEQ ID NO: 1 under the stringent condition and the length of the isolated deoxyribonucleic acid that does not hybridize to exon SV under the stringent condition hybridizes to exon 3 of SEQ ID NO: 1 under the stringent condition, wherein the isolated deoxyribonucleic acid includes a fluorescent label, a biotin label, a radioactive label, or an enzyme label and is 25 to 35 nucleotides in length. |
地址 |
Chiba JP |