| 摘要 |
The invention provides methods for screening potential therapeutic compounds useful in the treatment of metabolic diseases, e.g., diabetes. In particular, the invention provides methods of identifying compounds which modulate the activity of a mitochondrial anion carrier (MAC) which include contacting assay vesicles of the present invention with test compounds, determining the ability of the test compounds to modulate the transport of a MAC substrate across the lipid bilayer of the assay vesicle, and identifying the test compound as a modulator of MAC activity. The assay vesicles of the present invention include, for example, one two or three MACs, wherein the activity of at least one MAC is coupled to a detectable readout (e.g., a change in membrane potential and/or pH). The invention also provides novel assay vesicles as well as novel methods of preparing assay vesicles. |
