Manipulation of topography of large-format glass slides

摘要

System and method for correcting a topography of an object being imaged by a multi-array microscope system. The object is forced to conform to a surface of the substrate supporting the object with a suction force and the topography of the chosen object surface is determined. The supporting substrate is bent with the use of force applied to the substrate with at least one actuator such as to reduce the deviations of the determined topography of the object's surface from a pre-determined reference surface by transferring the changes in the curvature of the supporting substrate to the object. In particular, the chosen surface of the object can be substantially flattened for ease of simultaneous imaging of this surface with multiple objectives of the multi-array microscope.

主权项

1. A method for correcting a topography of a surface of an object, the method comprising:
disposing the object on a first surface of a supporting substrate, the supporting substrate having a second surface opposite to the first surface, the supporting substrate having a fluid port and structured to fluidly connect the first surface with the port through a body of the supporting substrate; measuring the object to determine differences between the topography of an upper surface of the object and a reference surface; and deforming the supporting substrate to change curvatures of both the first and second surfaces and to reduce said differences.