| 摘要 |
The method comprises (a) contacting the enzyme with a compound RpX, where R is 3'-nucleosidyl, p is a phospho radical and X is H or an esterifiable moiety, whereby ROH and pX are produced and (b) detecting ROH or pX. RpX may particularly be NAD3P - alternatively, X may be derived from riboflavin, pyridoxal, pyridoxamine, thiamine or a 1,2-dioxetane. Detection may be effected by fluorometric, colorimetric or luminometric means or via enzyme cycling or apoenzyme reactivation assays. The nuclease to be detected may be free in solution, immobilised on a surface or attached to a member of a specific binding pair and the method may thus be applied as a detection step in hybridisation assays, enzyme immunoassays or ligand:receptor binding assays.
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