| 摘要 |
Methods are provided that are useful in assaying compounds for cognitive enhancing properties, anxiolytic properties, hypnotic properties, or antidepressant properties. These methods involve determining the <i>in vitro</i> efficacy and EC50 of the compounds at defined series of cloned GABAA subtype receptors composed of specific variants of alpha , beta , and gamma subunits in order to develop and an activity profile for each compound. Optionally, the binding affinities of the compounds at GABAA receptors are also determined. As an additional step the <i>in vivo</i> effects of the compounds may be tested in animal models. |
