| 摘要 |
<p>NEW MATERIAL:A monoclonal antibody specifically recognizing the propeptide (profragment) of human prorenin. USE:A reagent for immunologically measuring or purifying the human prorenin. PREPARATION:For example, a human prorenin propeptide synthesized by a solid phase method is mixed with a Freund's perfect adjuvant and administered into the abdominal cavity of a BALB/c mouse to immunize the mouse. Three days later, splenic cells are aseptically collected and subsequently fused with the myeloma cells of a mouse, followed by culturing the fused cells in a HAT medium to multiply only the hybridoma. The hybridoma is identified by an ELISA method to screen a clone producing the desired antibody, which is cloned, followed by culturing the single clone to provide the monoclonal antibody.</p> |
