| 摘要 |
This invention relates to enzymes involved in alkaloid, and specifically nicotine, formation in tobacco plants. The invention is based, at least in part, on the nucleotide sequences encoding four variants of putrescine N-methyltransferase (PMT1, PMT2, PMT3, and PMT4), two variants of arginine decarboxylase (ADC1 and ADC2), ornithine decarboxylase (ODC), S-adenosylmethionine synthetase (SAMS), a fragment of NADH dehydrogenase, and a fragment of phosphoribosylanthranilate isomerase. The invention also relates to proteins expressed by these nucleotides, promoter regions of these nucleotides, use of these promoter regions to culture transgenic plant cells and to produce transgenic plants, sense and antisense nucleotides complementary to all or portions of these nucleotide sequences, use of sense and antisense nucleotides to regulate gene expression, and assays using proteins involved in alkaloid formation in tobacco plants. |
