Induction of the formation of insulin-producing cells via gene transfer of pancreatic beta-cell-associated transcriptional factor

摘要

The present invention provides a method of inducing the formation of insulin-producing cells which comprises transferring a pancreatic beta-cell associated transcriptional factor gene into the pancreas to induce the formation of insulin-producing cells. The pancreatic beta-cell associated transcriptional factor gene is transferred into the pancreatic tissue stem cells by the ICBD injection without ligating the common bile ducts and thus the formation of insulin-producing cells is induced. In the present invention, pdx-1, neurogenin3, etc. are used as such pancreatic beta-cell associated transcriptional factor gene and an adenoviral vector with the use of the Cre-loxP recombination system, etc. is used as a vector for transferring the pancreatic beta-cell associated transcriptional factor gene into the pancreas. The method of the invention enables regeneration therapy for diabetes mellitus by inducing the formation of insulin-producing cells.