Membrane stabilizing compositions and methods

摘要

A sugar-anchor polymer and methods of making and using the same to stabilize a lipid membrane. The sugar-anchor polymer includes a sugar covalently bound to a first anchor and a second anchor wherein the covalent bonds are selected from the group consisting essentially of an oxime bond, a hydrazone bond, an acylhydrazide bond, an aminothioacetal bond, an acetal bond, a thioacetal bond, a dithioacetal bond, a thioether bond and combinations thereof. The method includes reacting a sugar having at least two nucleophilic moieties with at least two anchors, each anchor having at least one electrophilic moiety, to form the polymer. Alternatively, the method includes reacting a sugar having at least two electrophilic moieties with at least two anchors, each anchor having at least one nucleophilic moiety, to form the polymer. The anchors may be lipids or peptides. The sugar-anchor polymers stabilize lipid membranes against serum proteins, dehydration, and cryopreservation.

主权项

1. A surface stabilized synthetic membrane comprising:
at least one lipid bilayer; and at least one sugar-anchor polymer coupled to the lipid bilayer by the anchor wherein the sugar-anchor polymer includes a first sugar having a first covalent bond to a first anchor and a second covalent bond to a second anchor, and a second sugar having a third covalent bond with at least one of the first anchor or the second anchor, wherein the first anchor and the second anchor are selected from the group consisting of a lipid and a peptide, and wherein the first, second, and third covalent bonds are selected from the group consisting of an oxime bond, a hydrazone bond, an acylhydrazide bond, an aminothioacetal bond, an acetal bond, a thioacetal bond, a dithioacetal bond, a thioether bond and combinations thereof.