MICROORGANISMS AND METHODS FOR PRODUCING 2,4-PENTADIENOATE, BUTADIENE, PROPYLENE, 1,3-BUTANEDIOL AND RELATED ALCOHOLS

摘要

The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non-naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.

主权项

1. A non-naturally occurring microbial organism, comprising at least two exogenous nucleic acids encoding 1,3-butanediol pathway enzymes expressed in a sufficient amount to convert malonyl-CoA and acetyl-CoA to 1,3-butanediol wherein at least one exogenous nucleic acid encodes an acetoacetyl-CoA synthase, catalyzing conversion of malonyl-CoA and acetyl-CoA to acetoacetyl-CoA, and wherein said microbial organism further comprises a 1,3-butanediol pathway selected from:
(1) 7P, 7N and 7AA; (2) 7I, 7G and 7S; (3), 7K, and 7S; (4) 7I, 7R and 7AA; (5) 7E, 7Q, 7Z and 7AA; (6) 7P, 7Y, 7Z and 7AA; (7) 7P and 7O; (8) 7E, 7F, 7R, and 7AA; and (9) 7E, 7F, 7G, and 7S, wherein 7E is an acetoacetyl-CoA hydrolase, acetoacetyl-CoA transferase or acetoacetyl-CoA synthetase catalyzing conversion of acetoacetyl-CoA to acetoacetate, wherein 7F is an acetoacetate reductase (acid reducing) catalyzing conversion of acetoacetate to 3, wherein 7G is a 3-oxobutyraldehyde reductase (aldehyde reducing) catalyzing conversion of 3-oxobutyraldehyde to 4-hydroxy-2-butanone, wherein 7I is an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming) catalyzing conversion of acetoacetyl-CoA to 3-oxobuytraldehyde, wherein 7K is an acetoacetyl-CoA reductase (alcohol forming) catalyzing conversion of acetoacetyl-CoA to 4-hydroxy-2-butanone, wherein 7N is a 3-hydroxybutyryl-CoA reductase (aldehyde forming) catalyzing conversion of 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde, wherein 7O is a 3-hydroxybutyryl-CoA reductase (alcohol forming) catalyzing conversion of acetoacetate to 3-hydroxybutyrate, wherein 7P is an acetoacetyl-CoA reductase (ketone reducing) catalyzing conversion of acetoacetyl-CoA to 3-hydroxybutyryl-CoA, wherein 7Q is an acetoacetate reductase (ketone reducing) catalyzing conversion of acetoacetate to 3-hydroxybutyrate, wherein 7R is a 3-oxobutyraldehyde reductase (ketone reducing) catalyzing conversion of 3-oxobutyraldehyde to 3-hydroxybutyraldehyde, wherein 7S is a 4-hydroxy-2-butanone reductase catalyzing conversion of 4-hydroxy-2-butanone to 1,3-butanediol, wherein 7Y is a 3-hydroxybutyryl-CoA hydrolase, a 3-hydroxybutyryl-CoA transferase or a 3-hydroxybutyryl-CoA synthetase catalyzing conversion of 3-hydroxybutyryl-CoA to 3-hydroxybutyrate, wherein 7Z is a 3-hydroxybutyrate reductase catalyzing conversion of 3-hydroxybutyrate to 3-hydroxybutyraldehdye, and wherein 7AA is a 3-hydroxybutyraldehyde reductase catalyzing conversion of 3-hydroxybutyraldehyde to 1,3-butanediol.