TUMOR SELECTIVE ANTIBODIES SPECIFIC TO ONCOFETAL ANTIGEN/IMMATURE LAMININ RECEPTOR PROTEIN

摘要

Disclosed are high affinity antibodies or antigen binding fragments thereof, which bind an epitope that lies within the C terminal region of oncofetal antigen (OFA)/immature laminin receptor protein (iLRP), and which do not substantially cross-react with mature OFA/LRP. The antibodies may be conjugated to cytotoxic moieties to enhance their therapeutic efficacy. Methods of making the antibodies and therapeutic and diagnostic uses thereof are also disclosed.

主权项

1. An antibody or a single-chain or antigen-binding fragment thereof that specifically binds a C-terminal epitope of oncofetal antigen (OFA)/immature laminin protein (iLRP) present on a tumor/cancer cell and which does not substantially cross-react with mature OFA/LRP or with non-cancer cells, wherein the antibody comprises a) three heavy chain complementarity determining regions (CDRs) which have the sequences GYTFTSYNMH, YIYPGNGGTNYNQKFKG, and GGYYYGSSWELYFDY, and three light chain CDRs which have the sequences RSSQSIVHSNGNTYLE, KVSNRFS, and FQGSHVPPT; b) three heavy chain CDRs which have the sequences GFSLTAYGVN, MIWGNGDTDYNSALKS, and YGY, and three light chain CDRs which have the sequences KSSQSLLDSDGKTYLN, LVSKVDS, and WQGTHFPFT; c) three heavy chain CDRs which have the sequences GFTFSSYTMS, TISSGGTYTYYPDSVKG, and LRY, and three light chain CDRs which have the sequences KSGQSLLDSDGKTYLN, LVSKLDS, and WQGTHFPQT; or d) three heavy chain CDRs which have the sequences GFSLTSYDIS, VIWTGGGTNYNSAFMS, and SFVY, and three light chain CDRs which have the sequences RSSQSLVHSNGNTYLH, KVSNRFS, and SQSTHVPWT, or a variant of any of said CDRs.