Integrated microfluidic device for single oocyte trapping

摘要

An integrated microfluidic device and its usage are provided. The microfluidic device comprises an upper layer (1) and a lower layer (2), wherein the lower layer (2) is bound to the upper layer (1). The upper layer (1) comprises a micro-channel (3) and the lower layer (2) comprises a micro-well (7) array. The micro-channel (3) is in fluidic connection with the micro-well (7) array, and the height of the micro-channel (3) is greater than the diameter of the oocyte (4) flowing through the micro-channel (3). The integrated microfluidic device has many advantages including low cost, high integration, and convenient operation, and has application prospects in reproductive medicine and the research of fertilization and embryo early development.

主权项

1. A method for single oocyte trapping using an integrated microfluidic device, the method comprising:
a) providing an integrated microfluidic device for single oocyte trapping, wherein the integrated microfluidic device comprises an upper layer and a lower layer, wherein said lower layer is bound to said upper layer, said upper layer comprises a micro-channel and said lower layer comprises a micro-well array comprising a plurality of micro-wells, said micro-channel is in fluidic connection with said micro-well array, and the height of said micro-channel is greater than the diameter of a mature mammalian oocyte; b) transporting an oocyte by a liquid in the micro-channel to allow the oocyte to enter the micro-well array; c) adding a sperm to the micro-channel to allow the sperm to reach the oocyte for in vitro fertilization in the micro-well array; and d) after the sperm reaches the oocyte, introducing air into the micro-channel to replace the liquid, so that micro-droplets are formed in the micro-wells.