| 摘要 |
<p>A kit for the quantitation of mRNA expression levels in a biological sample is provided which contains a detergent-chaotrope lysis mixture for extracting total RNA from the biological sample; a mixture of reverse transcriptase, nucleotides and an oligo-dT primer to synthesize cDNA from polyadenylated mRNA in the total mRNA; a means for labeling a primer or probe with a molecule which binds to an agent coated on a microtiter plate; a microtiter plate coated with an agent capable of binding to said molecule; a means for labeling a primer or probe with a detectable label; an antibody which detects the labeled primer or probe; and a means for detecting the antibody. Methods for quantitating mRNA expression in a biological sample are also provided.</p> |
