METHOD OF IDENTIFYING A NUCLEOTIDE PRESENT AT A DEFINED POSITION IN A NUCLEIC ACID

摘要

A method is described for identifying a nucleotide at a defined point on a nucleic acid sequence. An oligonucleotide probe is annealed to a target nucleotide sequence of the nucleic acid sample at a point immediately adjacent and 3' to the nucleotide of interest. The probe is then extended in the direction of the nucleotide of interest in a reaction medium containing at least one chain terminating nucleotide triphosphate (ATP, GTP, TTP and CTP). The nucleotide of interest is complementary to the labeled nucleotide incorporated into the primer by the extension reaction.