METHODS FOR TRANS-DESTABILIZATION OF SPECIFIC PROTEINS IN VIVO

摘要

This invention pertains to the metabolic destabilization a protein or peptide of interest in vivo. The protein or peptide of interest contains a second determinant of the N-end rule-based degradation signa l. The methods of the invention comprise contacting the protein or peptide of interest with a targeting protein or peptid e which interacts specifically with the protein or peptide of interest. The targeting peptide or protein contains a destabilizing amino-ter minal amino acid according to the N-end rule of protein degradation, but lacks a second determinant of the N-end rule-based degr adation signal. A requirement of the methods is that it is necessary to identify a peptide or protein which interacts specifical ly with the protein or peptide of interest. Because nearly all proteins specifically interact with other proteins, this is a broadly applicable method for metabolically destabilizing a protein or peptide of interest in vivo.