GLUCOSE RESPONSIVE INSULIN SECRETING BETA-CELL LINES AND METHOD FOR PRODUCING SAME

摘要

The invention pertains to a method of selecting cells which have enhanced secretion of secretory products by exposing a population of cells to a secretagogue in concentrations sufficient to form the secretory products. In particular, the method involves the selection of insulin-secreting beta-cells which have been exposed to the secretagogue, glucose, at an effective concentration. The selection process is accomplished by employing a fluorescence-activated cell sorter for the cells which have been marked with a calcium-activated labelling agent.