| 摘要 |
The detection system includes a pair of electrochemical hydrogen peroxide sensors, each sensor having working, counter and reference electrodes. A bias voltage is applied to maintain a voltage difference between the working and reference electrodes. A sample aliquot of fluid was treated with either sodium azide or catalase. The sensors are placed in containers containing sufficient amounts of treated fluid to cover the active portions of the electrodes. The output current of each sensor is amplified, and the resulting amplified signals are combined and subtracted to provide a signal which is representative of the level of hydrogen peroxide in the fluid. In a method for assessing oxidative stress, including that related to essential hypertension, the detection system is used to determine a representative level of hydrogen peroxide in blood plasma drawn from a test subject. The level of hydrogen peroxide is directly related to the level of reactive oxygen species in the plasma, and can be used as an accurate predictor of risk for essential hypertension or other conditions related to oxidative stress. |
