| 摘要 |
A method of performing polymerase chain reaction in a microfluidic device is provided. The microfluidic device has a central axis of rotation, a central reservoir, one or more radially-arranged channels, and one or more detection chambers or distal chambers in fluid communication with a respective one of the one or more radially-arranged channels. The method comprises the steps of: loading a liquid containing a sample into the central reservoir of the microfluidic device; spinning the microfluidic device about the central axis of rotation, thereby filling the one or more radially-arranged detection chambers or distal chambers with the liquid; and heating and cooling a portion of the microfluidic device, thereby performing polymerase chain reaction in the detection chambers or distal chambers. |
