发明名称 |
Treatment of methylated nucleic acid |
摘要 |
The invention provides methods for treating methylated nucleic acids. In one embodiment, the method can include the steps of (a) providing an alkali environment to a nucleic acid sample; (b) reacting the nucleic acid sample with a bisulphite reagent and incubating the reaction so as to form a treated nucleic acid sample where methylated nucleotides in the nucleic acid sample remain unchanged while unmethylated nucleotides are converted to another form; (c) diluting the treated nucleic acid sample so as to reduce salt concentration to a level which will not substantially interfere with a nucleic acid precipitating step; (d) precipitating the diluted treated nucleic acid to substantially remove any unwanted reagents or diluents from treated nucleic acid; and (e) carrying out de-sulphonation of the precipitated treated nucleic acid so as to remove sulphonate groups present on the treated nucleic acid so as to obtain a nucleic acid sample substantially free of sulphonate groups.
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申请公布号 |
US7288373(B2) |
申请公布日期 |
2007.10.30 |
申请号 |
US20030428310 |
申请日期 |
2003.05.02 |
申请人 |
HUMAN GENETIC SIGNATURES PTY LTD. |
发明人 |
MILLAR DOUGLAS SPENCER;VOCKLER CASSANDRA JEAN;COULSTON NERALIE ANN |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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