| 摘要 |
1523259 Sporamycin KITASATO INSTITUTE 6 July 1977 [10 July 1976] 28299/77 Heading C3H The antitumour antibiotic sporamycin having the following properties: (1) elemental analysis: carbon 44À72-47À38%; hydrogen 6À4-6À88%; nitrogen 13À9-15À0%; (2) ultraviolet absorption spectrum: absorption peaks at 285 nm. (shoulder), 276 nm,. 267 nm., 265 nm., 258 nm. and 253 nm. in an aqueous and 0À1 N hydrochloric acid solution; peak at 290 nm. in 0À1 N aqueous sodium hydroxide solution; (3) infrared absorption spectrum: absorption peaks at 3320 cm<-1>, 1640 cm.<-1> and 1520 cm.<-1> in KBr; (4) white powder; (5) solubility: soluble in water; insoluble in methanol, ethanol and acetone; (6) colour reaction: positive for Folin- Lowry, Ehrlich and biuret reactions; weakly positive for ninhydrin reaction; negative for Molisch, Fehling, Tollens and anthrone reactions; (7) molecular weight: 10,500-13,000; (8) amino acid analysis of 6N hydrochloric acid hydrolysis product (110‹ C.; 15 hours) : at least 12 kinds of amino acids: lysine, aspartic acid, threonine, serine, glutamic acid, glycine, alanine, valine, isoleucine, leucine, tyrosine and phenylalanine; (9) basic substance; (10 melting point: becomes brown at 220-225‹ C. and carbonizes with increasing temperature; and (11) [alpha]D<20>: approximately -56‹ (C=0À5, H2O) is claimed per se. It may be prepared by culturing Streptosporanjuim pseudovulgare in a conventional nutrient medium.
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