| 摘要 |
<p>An immunopurification process for separating an antigen from a sample containing the antigen involves an affinity matrix comprising a solid phase to which is attached an antibody which exhibits a positive temperature-dependent affinity such that the affinity of the antibody is substantially higher at a first relatively higher temperature than at a second relatively lower temperature. The process involves contacing the sample with the affinity matrix at the first temperature, causing antigen in the sample to become associated with the affinity matrix, removing unbound species from the affinity matrix and dissociating the antigen from the affinity matrix at the second temperature, to obtain the antigen. The process reduces the need for a chemical reagent to cause dissociation, thereby reducing the risk of denaturation of the antibody and/or the antigen. Examples are given in which the antigens are blood factor VIII and thrombin-antithrombin.</p> |
