| 摘要 |
PURPOSE:To prepare an interferon originated from human diploid fibroblast, in high purity, concentration, and yield, by specific multi-stage concentration purification process. CONSTITUTION:An aqueous solution of crude interferon originated from human fibroblast is passed through a column containing a strongly acidic cation exchange material [sulfo(propyl) sephadex, etc.] under acidic conditions to adsorb the interferon. The adsorbed interferon is eluted with a neutral or weak basic buffer solution, and passed through a column containing an insoluble carrier having zinc iminodiacetate (e.g. obtained by bonding iminodiacetic acid to an epoxy-activated sepharose, and converting the free acid to zinc salt). The adsorbed interferon is eluted and recovered with a weak acidic solution.
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