Filter systems for separating microcarriers from cell culture solutions

摘要

A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

主权项

1. A filter system for separating microcarriers from a fluid medium, the filter system comprising:
a substantially rigid support housing having a floor with a side wall upstanding therefrom, the floor and the side wall bounding a chamber; and a filter assembly being removably disposable within the chamber of the support housing, the filter assembly comprising:
a container bounding a sterile compartment adapted to hold a fluid;an inlet port through which fluid flows into the compartment, the inlet port being removably attachable to the support housing;an outlet port through which fluid flows out of the compartment; anda filter disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough, the filter comprising a porous bag or sock that bounds the inlet chamber, the porous bag or sock being coupled with the inlet port either directly or through a tube.