Method for treating a blood component containing sample

摘要

Provided is a treatment method for damaging an erythrocyte and a leukocyte while suppressing damage to cells other than blood cells present in blood. In an embodiment, the disclosure relates to a method for treating a sample containing blood components, the method including mixing a sample containing blood components with a surfactant A, where the surfactant A is a nonionic surfactant represented by General formula R1—O-(EO)n-R2 (I).

主权项

1. A method for separating or detecting a tumor cell and/or a cancer cell in a sample containing blood components, the method comprising treating the sample by mixing the sample with a liquid containing a surfactant A and a liquid containing a surfactant B, or with a liquid containing the surfactant A and the surfactant B,
wherein the surfactant A is a nonionic surfactant of formula (I)
R1—O-(EO)n—R2  (I) wherein: R1 is a hydrocarbon group having a branched chain and a carbon number in the range of 12 to 40, EO is an oxyethylene group, n is an average addition mole number of EO in the range of 23 to 50, and R2 is a hydrogen atom or a hydrocarbon group having a carbon number in the range of 1 to 3; and the surfactant B is a nonionic surfactant selected from the group consisting of a sorbitan fatty acid ester, a compound of formula (III), a compound of formula (IV), a compound of formula (VI) and combinations thereof,
R3—O-(AO)m/(EO)n—R4  (III) wherein: R3 is an alkyl group having a carbon number in the range of 1 to 24, R4 is a hydrogen atom or an alkyl group having a carbon number in the range of 1 to 3, AO is an oxyalkylene group having a carbon number in the range of 3 to 6, EO is an oxyethylene group, m and n are average addition mole numbers of AO and EO respectively, where m is a number in the range of 1 to 100 and n is a number in the range of 0 to 50, and “/” indicates that the AO group and the EO group may be added at random or as a block regardless of the order;
R5—O-(EO)n—R6  (IV) wherein: R5 is a hydrocarbon group having a carbon number in the range of 12 to 40, EO is an oxyethylene group, n is an average addition mole number of EO in the range of 8 to 22, and R6 is a hydrogen atom or a hydrocarbon group having a carbon number in the range of 1 to 3;
R7—COO-(EO)n—R8  (VI) wherein: R7 is a hydrocarbon group having a carbon number in the range of 10 to 40, EO is an oxyethylene group, n is an average addition mole number of EO in the range of 6 to 160, and R8 is a hydrogen atom or a hydrocarbon group having a carbon number in the range of 1 to 3, where the surfactant B has a lytic property with respect to an erythrocyte higher than the lytic property of the surfactant A with respect to an erythrocyte, and wherein the method eliminates erythrocytes and leukocytes present in the sample while suppressing damage to the tumor cell and/or the cancer cell.