发明名称 |
PRODUCTION OF POLYPEPTIDE HAVING HUMAN INTERLEUKIN 2 ACTIVITY |
摘要 |
PURPOSE:To obtain the title polypeptide free from other proteins originating from human and from saccharides by culturing procaryotic cells transformed with a specific recombinant DNA. CONSTITUTION:Procaryotic cells, preferably in Escherichia coli are transformed with a recombinant DNA which includes the genes coding (a) a promoter, (b) a ribosome-binding site, (c) a translation-starting codon and (d) a polypeptide having human interleukin 2 activity in this order from the upperstream of plasmid vector DNA chain which is compatible to procaryotic cells, and cultured aerobically in suspension. |
申请公布号 |
JPH0335795(A) |
申请公布日期 |
1991.02.15 |
申请号 |
JP19890109058 |
申请日期 |
1989.05.01 |
申请人 |
JAPAN FOUND CANCER RES;AJINOMOTO CO INC |
发明人 |
TANIGUCHI KOREAKI;MURAMATSU MASAMI;SUGANO HARUO;MATSUI YUTAKA;KASHIMA SHINICHI;HAMURO JUNJI |
分类号 |
C12N1/21;A61K35/12;A61K35/14;A61K35/74;A61K38/00;A61P35/00;A61P37/00;C07H21/02;C07H21/04;C07K1/22;C07K14/00;C07K14/52;C07K14/54;C07K14/55;C12N15/00;C12N15/09;C12N15/26;C12N15/70;C12P21/00;C12P21/02;C12R1/19;C12R1/91 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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