摘要 |
A DNA sequence containing a ribosome binding site with a SD sequence AGGA and a start codon ATG, comprising a nucleotide sequence having the formula Y1 . . . YmZVAGGA X1, X2 . . . XnATG wherein Y1 . . . Ym represents a promoter with one or more restriction enzyme sites, m being an integer corresponding to the number of base pairs in the promoter, each X, Y, Z and V is A, T, C or G; n is 9 to 13, and the sequence X1 . . . Xn cannot contain a start codon.
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