| 摘要 |
The present invention provides methods to identify acceptor germline sequences for a humanized antibody, which methods comprise the steps of:
a) identifying a non-human antibody that has the desired biological activity;
b) determining the amino acid sequence of a non-human antibody V H and V L domains; and
c) comparing the nonhuman antibody sequence to a group of human germline sequences, wherein the comparison comprises the substeps of:
(1) assigning the sequence of non-human V H and V L domain sequences residue numbers;
(2) delineating the CDR and FR regions in the sequence;
(3) assigning a predetermined numerical score at each residue position for which the non-human and human germline sequences are identical; and
(4) totaling all of the residue scores to generate a total score for each human germline sequence; and
d) identifying the human germline sequence with the highest total residue score as the acceptor germline sequence. |
