| 摘要 |
TPA-DHD can be produced by using, as a raw material, terephthalic acid salt that contains 0.5 times or more and twice or less the amount of potassium based on all of the terephthalic acids contained in the terephthalic acid salt in terms of moles and by using a microorganism expressing terephthalate 1,2-dioxygenase. Further, TPA-DHD can be converted into protocatechuic acid by TPA-DHD dehydrogenase and protocatechuic acid can be converted into gallic acid by para-hydroxybenzoate hydroxylase. In addition, by subjecting waste polyesters to heat treatment in an ethylene glycol solvent or 1-butanol solvent containing potassium hydroxide, such polyesters can be efficiently depolymerized, and potassium terephthalate suitable to chemical production by the microorganism can be prepared. |
| 主权项 |
1. A method of producing terephthalate 1,2-cis-dihydrodiol or metabolite thereof comprising reacting a microorganism or a processed product of a culture thereof with terephthalic acid in an aqueous medium containing a terephthalic acid salt to generate terephthalate 1,2-cis-dihydrodiol or metabolite thereof, wherein said terephthalic acid salt comprises terephthalic acid potassium salt and the molar amount of potassium in the terephthalic acid salt is between 0.5 times and 2 times the molar amount of the terephthalic acid contained in the terephthalic acid salt, wherein said microorganism has the ability to produce terephthalate 1,2-cis-dihydrodiol from terephthalic acid and comprises three DNAs selected from the group consisting of (a), (b), (c), (d), (e), (f), (g), (h), and (i), wherein the first DNA is any one of the DNAs set forth in (a), (b), or (c), wherein the second DNA is any one of the DNAs set forth in (d), (e), or (f), wherein the third DNA is any one of the DNAs set forth in (g), (h), or (i), and wherein the DNAs of (a) to (i) are:
(a) a DNA coding for a protein comprising the amino acid sequence set forth in SEQ ID NO: 2; (b) a DNA coding for a protein having the activity of a terephthalate 1,2-dioxygenase oxygenase large subunit protein which is involved in the conversion of terephthalic acid into terephthalate 1,2-cis dihydrodiol, wherein said protein comprises an amino acid sequence at least 95% identical to the whole amino acid sequence set forth in SEQ ID NO: 2; (c) a DNA comprising the nucleotide sequence set forth in SEQ ID NO: 1; (d) a DNA coding for a protein comprising the amino acid sequence set forth in SEQ ID NO: 4; (e) a DNA coding for a protein having the activity of a terephthalate 1,2-dioxygenase oxygenase small subunit protein which is involved in the conversion of terephthalic acid into terephthalate 1,2-cis dihydrodiol, wherein said protein comprises an amino acid sequence at least 95% identical to the whole amino acid sequence set forth in SEQ ID NO: 4; (f) a DNA comprising the nucleotide sequence set forth in SEQ ID NO: 3; (g) a DNA coding for a protein comprising the amino acid sequence set forth in SEQ ID NO: 6; (h) a DNA coding for a protein having the activity of a terephthalate 1,2-dioxygenase reductase protein which is involved in the conversion of terephthalic acid into terephthalate 1,2-cis-dihydrodiol, wherein said protein comprises an amino acid sequence at least 95% identical to the whole amino acid sequence set forth in SEQ ID NO: 6; and (i) a DNA comprising the nucleotide sequence set forth in SEQ ID NO: 5. |
