Compositions and methods for capture and elution of biological materials via particulates

摘要

A flow-through device for capturing a biological material from a fluid specimen and lysing the fluid specimen. The flow through device may include a first chamber containing a portion of the fluid specimen and a solid phase material retained in the first chamber. The solid phase material may have an affinity for the biological material in the fluid specimen. The first chamber may include an inlet opening which allows flow of the fluid specimen into the first chamber. The first chamber may also include an outlet opening which allows flow of the fluid specimen out of the first chamber. The flow through device may include a second chamber fluidly connected to the first chamber. The second chamber may include a portion of the fluid specimen and an impeller. The impeller may in use impart rotational motion to the fluid specimen to mechanically lyse the fluid specimen.

主权项

1. A flow-through device for capturing a biological material from a fluid specimen and lysing the fluid specimen, the device comprising:
a first chamber containing: a portion of the fluid specimen; a solid phase material retained in the first chamber, the solid phase material having an affinity for the biological material in the fluid specimen; wherein the first chamber comprises: an inlet opening allowing flow of the fluid specimen into the first chamber; a first fitting attached to the inlet opening of the first chamber, the first fitting having a first thin filter insert that retains the solid phase material while allowing rapid flow of the fluid specimen at low pressure; an outlet opening allowing flow of the fluid specimen out of the first chamber; and a second fitting attached to the outlet opening of the first chamber, the second fitting having a second thin filter insert that retains the solid phase material while allowing rapid flow of the fluid specimen at low pressure; and a second chamber fluidly coupled to the first chamber, the second chamber containing: a portion of the fluid specimen; wherein the second chamber comprises: an impeller which in use imparts rotational motion to the fluid specimen to mechanically lyse the fluid specimen.