CHEMICALLY DEFINED MEDIUM FOR HUMAN MESENCHYMAL STEM CELLS

摘要

A composition and method for maintaining the viability of human mesenchymal precursor cells in a serum-free environment which composition includes: (1) a minimum essential medium; (2) serum albumin; (3) an iron source; (4) insulin or an insulin-like growth factor; and (5) at least one amino acid selected from the group consisting of glutamine, arginine and cysteine, and is free of serum. Also, a composition and method for culture expanding human mesenchymal precursor cells in a serum-free environment. This composition further includes a mitogen, particularly a serotonergic agonist. The cells are preferably isolated human mesenchymal stem cells.