BIOTIN-LABELLED DNA BY POLYMERASE CHAIN REACTION AND DETECTION THEREOF

摘要

A format for hybridization capture of PCR amplified DNA on a solid support is provided. After labelling the amplified target DNA with biotin during amplification, the labelled DNA is specifically captured by base-pair hybridization to an amplicon-specific oligonucleotide capture probe which is bound to a solid support and the labeled DNA is detected with a biotin dependent chromogenic detection assay. In a specific format selected primers and probes are disclosed which enable the detection of Chlamydia trachomatis.