发明名称 Expression vector and method for the expression of heterologous proteins in mammalian cells.
摘要 <p>A vector which contains at least a first consensus sequence which is, in the first three nucleotides and at least in a total of 10 nucleotides, homologous with the sequence CTC@ GAGACCAA and at least a second consensus sequence which is, either in nucleotide 6, 8, 9 and 10 and in at least 9 nucleotides, homologous with the sequence TTTA@ ATTTTC, or in at least 10 nucleotides homologous with the sequence TATGATAATGAG, or homologous with the sequence TGG(N)6-7GCCAA and contains an inefficient selection system, is prepared as described. &lt;??&gt;Vectors of this type can be amplified quickly, can be transferred universally into mammalian cells and are free of viral origin sequences. </p>
申请公布号 EP0306848(B1) 申请公布日期 1993.11.03
申请号 EP19880114317 申请日期 1988.09.02
申请人 BOEHRINGER MANNHEIM GMBH 发明人 GRUMMT, FRIEDRICH, PROF. DR.;WEIDLE, ULRICH, DR. RER. NAT.
分类号 C12N15/09;C12N9/72;C12N15/85;C12P21/00;C12R1/91;(IPC1-7):C12N15/00 主分类号 C12N15/09
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