Method and apparatus for the on-location field detection of unidentified antigenic substances

摘要

A method and test device are disclosed for detecting under field conditions unidentified antigenic substances which are detrimental to human or animal health or welfare. A sample solution containing the suspected unidentified antigenic substance is added to the test device containing a known antigenic substance which is irreversibly attached to a solid support and suspended in a buffer solution along with an antibody specific to the known antigenic substance conjugated with a detecting agent. After incubation to bind the unknown antigenic substance to the conjugate, the solution contacts a white porous bibulous sheet incorporating a substrate for the detecting agent and a chromogen, which is part of the test device. If the identity of the sample antigen is the same as that on the solid support, a color reaction takes place.