PURIFICATION AND DETECTION PROCESSES USING REVERSIBLE AFFINITY ELECTROPHORESIS

摘要

An affinity electrophoresis process is described, in which the direction of electrophoresis is varied in a cyclical manner while synchronously changing one or more property of the electrophoretic medium between two states, said states being characterized as favoring or disfavoring specific reversible binding of sample analytes to affinity ligands which are immobilized within the medium. The resulting process enables extremely efficient and convenient separation of the specific analytes for detection or purification, using simple materials and apparatus.