| 摘要 |
Provided is a technique whereby, in the case of simultaneously analyzing gene expression of multiple cells at a single cell level, the use amount of an expensive reagent can be reduced, a lowering in reaction efficiency due to turbidity caused by reagents mixed together can be suppressed and a device can be effectively washed so as to protect mRNA from contamination with cells other than the target cells. The cell treatment device according to the present invention comprises: a reaction field in which an organism-derived sample is captured; a housing part in which the sample introduced from outside, a reagent or a washing liquor are temporarily stored; a first flow channel which connects the reaction field and the housing part; an opening through which the sample and the reagent are introduced from outside the device; a second flow channel which connects the opening and the housing part; an inlet through which the washing liquor is introduced from outside the device; and a third flow channel which connects the inlet and the second flow channel. In this cell treatment device, the opening, a joint part of the second flow channel and the third flow channel, and an outlet in the housing part toward the first flow channel are arranged in this order from top to bottom in the vertical direction. |
