| 摘要 |
<p>PROBLEM TO BE SOLVED: To obtain a paramyxovirus vector capable of transducing a foreign gene, having a replication ability. SOLUTION: A Sendai virus having a foreign gene is constructed by inserting the foreign gene into before or behind a virus gene of Sendai virus genome cDNA. The Sendai virus proves that it has a replication ability and a foreign gene is expressed in a transduced cell. The expression amount of the foreign gene is higher closer to 3' of negative chain RNA and a high expression is obtained when the foreign gene is inserted into especially before an NP gene and between the NP gene and a P gene. On the contrary, the expression is lower closer to 5' of the negative chain RNA and a relatively low expression is obtained when the foreign gene is inserted into especially behind an L gene, between a HN gene and the L gene and between an F gene and the NH gene.</p> |
