MEDIA FOR ISOLATION AND STABILIZATION OF CELLS

摘要

Viable microvessel endothelial cells are isolated from tissue in high yield by enzymatic digestion of tissue containing the cells using as a medium for the digestion, an enzyme composition in a physiologically acceptable aqueous electrolyte solution of pH 6.5 to pH 8.0 consisting essentially of sodium, potassium, magnesium, chloride, acetate, and gluconate ions, and having an osmolarity of about 270 mOsmol/L to 310 mOsmol/L. The electrolyte solution when mixed with a blood component is effective for stabilizing viable animal cells for periods of up to 4 hours.