Methods and compositions for separating nucleic acids from a solid support

摘要

Methods and compositions for separating nucleic acids from a solid support are provided. In the subject methods, a silica containing solid support having nucleic acids immobilized on a surface thereof is subjected to cleavage conditions, such that a fluid cleavage product that includes nucleic acids and silica is produced. The resultant fluid cleavage product is then purified to produce a final nucleic acid composition that includes a substantially reduced amount of silica. Also provided are kits for practicing the subject methods.