Biorefinery system, methods and compositions thereof

摘要

The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

主权项

1. A methanotroph bacteria, wherein said methanotroph bacteria comprises a heterologous polynucleotide encoding a thioesterase, a malonyl-CoA-acyl carrier protein transacylase, an acetyl-CoA carboxylase or any combination thereof, and wherein said methanotroph bacteria accumulates an increased level of fatty acids when grown on a C1 substrate as a carbon source when compared to a wild-type methanotroph bacteria without said heterologous polynucleotide and grown under same conditions.